Insulin Protein (INS) (GST tag)
Quick Overview for Insulin Protein (INS) (GST tag) (ABIN7668939)
抗原
See all Insulin (INS) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This Insulin protein is labelled with GST tag.
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原理
- Recombinant Mouse INS protein (GST tag)
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序列
- Phe 25-Asn 110
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纯化方法
- > 95 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 10 EU/mg of the protein as determined by the LAL method.
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Insulin (INS) protein
INS 宿主: 人 宿主: 酵母菌 Recombinant Greater than 95 % as determined by SDS-PAGE.
Insulin (INS) (AA 1-110) protein (GST tag)INS 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Insulin (INS) (AA 25-54), (AA 90-110) (Active) protein (His tag)INS 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Insulin (INS)
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别名
- INS
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背景
- Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.,IDDM,IRDN,Ins-2,Insulin B chain,Proinsulin,ins
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分子量
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calculated_mw: 34.4 kDa
observed_mw: 30 kDa
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UniProt
- P01326
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途径
- NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
抗原
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