1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human TNFSF11 at 2 μg/mL (100 μL/well) can bind Recombinant Mouse TNFRSF11B with a linear range of 3-10 ng/mL.|2.Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 0.13-0.54 ng/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
缓冲液
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存条件
-20 °C,-80 °C
储存方法
Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
抗原
Osteoprotegerin (TNFRSF11B)
(Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))