Leptin Receptor Protein (LEPR) (Fc Tag,His tag)
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- 抗原 See all Leptin Receptor (LEPR) 蛋白
- Leptin Receptor (LEPR)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Leptin Receptor protein is labelled with Fc Tag,His tag.
- 原理
- Active Recombinant Human Leptin receptor/LEP-R/CD295 Protein
- 序列
- TAFNLSYPIT PWRFKLSCMP PNSTYDYFLL PAGLSKNTSN SNGHYETAVE PKFNSSGTHF SNLSKTTFHC CFRSEQDRNC SLCADNIEGK TFVSTVNSLV FQQIDANWNI QCWLKGDLKL FICYVESLFK NLFRNYNYKV HLLYVLPEVL EDSPLVPQKG SFQMVHCNCS VHECCECLVP VPTAKLNDTL LMCLKITSGG VIFQSPLMSV QPINMVKPDP PLGLHMEITD DGNLKISWSS PPLVPFPLQY QVKYSENSTT VIREADKIVS ATSLLVDSIL PGSSYEVQVR GKRLDGPGIW SDWSTPRVFT TQDVIYFPPK ILTSVGSNVS FHCIYKKENK IVPSKEIVWW MNLAEKIPQS QYDVVSDHVS KVTFFNLNET KPRGKFTYDA VYCCNEHECH HRYAELYVID VNINISCETD GYLTKMTCRW STSTIQSLAE STLQLRYHRS SLYCSDIPSI HPISEPKDCY LQSDGFYECI FQPIFLLSGY TMWIRINHSL GSLDSPPTCV LPDSVVKPLP PSSVKAEITI NIGLLKISWE KPVFPENNLQ FQIRYGLSGK EVQWKMYEVY DAKSKSVSLP VPDLCAVYAV QVRCKRLDGL GYWSNWSNPA YTVVMDIKVP MRGPEFWRII NGDTMKKEKN VTLLWKPLMK NDSLCSVQRY VINHHTSCNG TWSEDVGNHT KFTFLWTEQA HTVTVLAINS IGASVANFNL TFSWPMSKVN IVQSLSAYPL NSSCVIVSWI LSPSDYKLMY FIIEWKNLNE DGEIKWLRIS SSVKKYYIHD HFIPIEKYQF SLYPIFMEGV GKPKIINSFT QDDIEKHQSD
- 特异性
- Thr20-Asp839
- 纯度
- > 95 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- < 1.0 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Leptin protein at 2 μg/mL (100 μL/well) can bind Recombinant Human Leptin R/CD295 protein,the EC50 of Leptin R/CD295 is 261.45 ng/mL.
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- Discover our top product LEPR 蛋白
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Leptin Receptor (LEPR) protein (Fc Tag)
LEPR 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin Receptor (LEPR) (AA 22-839) protein (His tag)LEPR 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Leptin Receptor (LEPR) protein (Fc Tag)LEPR 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin Receptor (LEPR) (AA 31-130) protein (GST tag)LEPR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Leptin Receptor (LEPR) protein (mFc Tag)LEPR 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin Receptor (LEPR) protein (His tag)LEPR 宿主: 小鼠 宿主: 人细胞 Recombinant >90 % as determined by reducing SDS-PAGE.
Leptin Receptor (LEPR) (AA 863-1165) protein (His tag)Crystallography grade LEPR 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leptin Receptor (LEPR) (AA 861-1162) protein (His tag)Crystallography grade LEPR 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leptin Receptor (LEPR) (AA 22-839) protein (His tag)Crystallography grade LEPR 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leptin Receptor (LEPR) (AA 22-839) protein (His tag)Crystallography grade LEPR 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of 50 mM MES, 100 mM NaCl,1 mM DTT, pH 6.5.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,-80 °C
- 储存方法
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- 抗原
- Leptin Receptor (LEPR)
- 别名
- Leptin receptor/LEP-R/CD295 (LEPR 产品)
- 别名
- CD295 Protein, LEP-R Protein, LEPRD Protein, OB-R Protein, OBR Protein, obr Protein, cd295 Protein, ob-rb Protein, Fa Protein, LEPROT Protein, Leprb Protein, Modb1 Protein, OB-RGRP Protein, Obr Protein, db Protein, diabetes Protein, obese-like Protein, obl Protein, ObRb Protein, Ob-R Protein, Ob-RM Protein, lepr Protein, leptin receptor Protein, leptin receptor S homeolog Protein, LEPR Protein, lepr Protein, Lepr Protein, lepr.S Protein
- 背景
- CD295,LEP-R,LEPRD,OB-R,OBR,LEPR
- 基因ID
- 3953
- UniProt
- P48357
- 途径
- JAK/STAT Signaling, AMPK Signaling, Feeding Behaviour
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