Leptin Protein (LEP)
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- 抗原 See all Leptin (LEP) 蛋白
- Leptin (LEP)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 原理
- Active Recombinant Human Leptin/LEP Protein
- 序列
- VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC
- 特异性
- Val22-Cys167
- 纯度
- > 92 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Leptin at 2 μg/mL (100 μL/well) can bind Recombinant Human Leptin R/CD295 with a linear range of 1.22-261.45 ng/mL.
- Top Product
- Discover our top product LEP 蛋白
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Leptin (LEP) (AA 22-167) protein (Fc Tag)
LEP 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leptin (LEP) (Active) proteinLEP 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Leptin (LEP) proteinLEP 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 22-167) protein (His tag)LEP 宿主: Pig 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Leptin (LEP) protein (His tag)LEP 宿主: Goldfish 宿主: 酵母菌 Recombinant > 95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 428-635) protein (His tag)LEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Leptin (LEP) (AA 2-147) proteinLEP 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Leptin (LEP) (AA 22-167) protein (GST tag)LEP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Leptin (LEP) (AA 29-164), (partial) protein (GST tag)LEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 8.0.
- 储存条件
- -20 °C,-80 °C
- 储存方法
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- 抗原
- Leptin (LEP)
- 别名
- Leptin/LEP (LEP 产品)
- 别名
- ob Protein, obese Protein, LEPD Protein, OB Protein, OBS Protein, leptin Protein, Lep Protein, LEP Protein, lep Protein
- 背景
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Description: Leptin is one of the most important hormones secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this protein and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This protein has also been linked to type 2 diabetes mellitus development.
Name: LEP,LEPD,OB,OBS,leptin
- 基因ID
- 3952
- UniProt
- P41159
- 途径
- JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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