IGFBP3 Protein (His tag)
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- 抗原 See all IGFBP3 蛋白
- IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This IGFBP3 protein is labelled with His tag.
- 原理
- Active Recombinant Human IGFBP-3 Protein
- 序列
- GASSAGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG SVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK
- 特异性
- Gly28-Lys291
- 纯度
- > 95 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- <1EU/μg
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Human IGFBP3 Protein at 1 μg/mL (100 μL/well) can bind IGF1 with a linear range of 0.158-23.05ng/mL.
- Top Product
- Discover our top product IGFBP3 蛋白
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Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (Active) protein
IGFBP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (AA 28-291) protein (His tag)IGFBP3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (AA 120-244) protein (His tag)IGFBP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (AA 28-291) protein (His tag)IGFBP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (AA 182-291) protein (GST tag)IGFBP3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (AA 28-291) protein (His tag)Crystallography grade IGFBP3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (AA 28-292) protein (His tag)Crystallography grade IGFBP3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- 抗原
- IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
- 别名
- IGFBP-3 (IGFBP3 产品)
- 别名
- BP-53 Protein, IBP3 Protein, AI649005 Protein, IGFBP-3 Protein, IGgfbp3 Protein, IGF-BP3 Protein, zgc:91788 Protein, IGFBP3 Protein, igfbp3 Protein, insulin like growth factor binding protein 3 Protein, insulin-like growth factor binding protein 3 Protein, IGFBP3 Protein, Igfbp3 Protein, igfbp3 Protein, IGFBP-3 Protein, LOC100305016 Protein
- 背景
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Description: The Insulin-like Growth Factor (IGF) signaling system plays a central role in cellular growth, differentiation, and proliferation. IGFBP3 is the most abundant IGF binding protein in human serum and is a growth inhibitory, apoptosis-inducing molecule, capable of acting via IGF-dependent and IGF-independent mechanisms. It appears to function both by cell cycle blockade and the induction of apoptosis. IGFBP3 can be transported to the nucleus by an importin beta mediated mechanism, where it has been shown to interact with the retinoid X receptor alpha and possibly other nuclear elements. IGFBP3 antiproliferative signaling appears to require an active transforming growth factor-beta (TGF-beta) signaling pathway, and IGFBP3 stimulates phosphorylation of the TGF-beta signaling intermediates Smad2 and Smad3. IGFBP3 has IGF-independent roles in inhibiting cell proliferation in cancer cell lines. Nuclear transcription factor, retinoid X receptor (RXR)-alpha, and IGFBP3 functionally interact to reduce prostate tumor growth and prostate-specific antigen in vivo. Several clinical studies have proposed that individuals with IGFBP3 levels in the upper range of normal may have a decreased risk for certain common cancers. This includes evidence of a protective effect against breast cancer, prostate cancer, colorectal cancer, and lung cancer. Moreover, IGFBP3 inhibits insulin-stimulated glucose uptake into adipocytes independent of IGF.
Name: IGFBP3,BP-53,IBP3
- 基因ID
- 3486
- UniProt
- P17936-1
- 途径
- Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
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