desert Hedgehog Protein (His tag)
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- 抗原 See all desert Hedgehog (DHH) 蛋白
- desert Hedgehog (DHH) (Desert Hedgehog (DHH))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This desert Hedgehog protein is labelled with His tag.
- 原理
- Recombinant Human HHG-3/DHH Protein
- 序列
- CGPGRGPVGR RRYARKQLVP LLYKQFVPGV PERTLGASGP AEGRVARGSE RFRDLVPNYN PDIIFKDEEN SGADRLMTER CKERVNALAI AVMNMWPGVR LRVTEGWDED GHHAQDSLHY EGRALDITTS DRDRNKYGLL ARLAVEAGFD WVYYESRNHV HVSVKADNSL AVRAGG
- 特异性
- Cys23-Gly198
- 过滤
- 0.22 μm filtered
- 内毒素水平
- <1 EU/μg
- Top Product
- Discover our top product DHH 蛋白
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Desert Hedgehog (DHH) (Active) protein
DHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Desert Hedgehog (DHH) (AA 24-198) protein (His tag)DHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Desert Hedgehog (DHH) (AA 24-198) protein (His tag)DHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Desert Hedgehog (DHH) (AA 50-320) protein (His tag)DHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Desert Hedgehog (DHH) (AA 1-396) protein (GST tag)DHH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Desert Hedgehog (DHH) (AA 23-198) protein (His tag)Crystallography grade DHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Desert Hedgehog (DHH) (AA 23-396) protein (His tag)Crystallography grade DHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Desert Hedgehog (DHH) (AA 199-396) protein (His tag)Crystallography grade DHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Desert Hedgehog (DHH) (AA 23-198) protein (His tag)Crystallography grade DHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Desert Hedgehog (DHH) (AA 23-396) protein (His tag)Crystallography grade DHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 浓度
- 0.4 mg/mL
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS,1 mM DTT, pH 7.4.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- 抗原
- desert Hedgehog (DHH) (Desert Hedgehog (DHH))
- 别名
- HHG-3/DHH (DHH 产品)
- 别名
- GDXYM Protein, HHG-3 Protein, SRXY7 Protein, C78960 Protein, Xchh Protein, chh Protein, chh-a Protein, dhh Protein, dhh-1 Protein, dhh-A Protein, hh4 Protein, x-chh Protein, desert hedgehog Protein, desert hedgehog L homeolog Protein, desert hedgehog S homeolog Protein, DHH Protein, dhh Protein, Dhh Protein, dhh.L Protein, dhh.S Protein
- 物质类
- Species
- 背景
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Description: Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Desert Hedgehog is a secreted, 45 kDa, 373 amino acid (aa) protein that undergoes autocatalytic cleavage between Gly198 and Cys199 catalyzed by the C-terminal domain, which releases the N-terminal domain with a concominant attachment of cholesterol at its new C-terminus. In addition to the C-terminally attached cholesterol, a fatty acid acyl chain is esterified to the N-terminal cysteine (aa 23) via an amide linkage. The 19 kDa N-terminal signaling domain is membrane associated due to its double lipid modifications. Its binding to Patched receptors results in the loss of Patched repression of Smoothened signaling (1 - 4). Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip) (5). Within the N-terminal domain, human Dhh shares 97 % aa sequence identity with mouse and rat Dhh. It shares approximately 75 % aa seqeuence identity with human Indian (Ihh) and Sonic Hedgehog (Shh) (5). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis (6, 7). It induces steroidogenic factor 1, which is instrumental in promoting Leydig cell differentiation (8, 9).
Name: GDMN, GDXYM, HHG-3, SRXY7,HHG-3,DHH
- 基因ID
- 50846
- UniProt
- O43323
- 途径
- Hedgehog Signaling
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