Recombinant Human BTN2A1 & BTN3A1 complex Protein is expressed from HEK293 with His tag and Flag tag at the C-terminus.It contains Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
MALS verified
BTN2A1
宿主: 人
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
BTN2A1
(butyrophilin, Subfamily 2, Member A1 (BTN2A1))
BK14H9.1 Protein, BT2.1 Protein, BTF1 Protein, DJ3E1.1 Protein, BTN2A1 Protein, btf1 Protein, bt2.1 Protein, BTN2A2 Protein, butyrophilin subfamily 2 member A1 Protein, butyrophilin subfamily 2 member A1 S homeolog Protein, butyrophilin, subfamily 2, member A1 Protein, BTN2A1 Protein, btn2a1.S Protein, btn2a1 Protein, LOC100409586 Protein
背景
BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells.
分子量
30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa (BTN2A1) and 33-38 kDa (BTN3A1) based on Tris-Bis PAGE result.