BTN2A1 Protein (Fc-Avi Tag,Biotin)
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- 抗原 See all BTN2A1 蛋白
- BTN2A1 (butyrophilin, Subfamily 2, Member A1 (BTN2A1))
- 蛋白类型
- Recombinant
- 宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This BTN2A1 protein is labelled with Fc-Avi Tag,Biotin.
- 原理
- Biotinylated Human BTN2A1 Protein, Fc,Avitag™ (MALS verified)
- 序列
- Gln 29 - Ala 248
- 产品特性
- Biotinylated Human BTN2A1, Fc,Avitag (BT1-H52F5) is expressed from human 293 cells (HEK293). It contains AA Gln 29 - Ala 248 (Accession # Q7KYR7-2).
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- 质量等级
- MALS verified
- Top Product
- Discover our top product BTN2A1 蛋白
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butyrophilin, Subfamily 2, Member A1 (BTN2A1) (AA 29-248) protein (His tag)
BTN2A1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
butyrophilin, Subfamily 2, Member A1 (BTN2A1) (AA 1-334) protein (GST tag)BTN2A1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
butyrophilin, Subfamily 2, Member A1 (BTN2A1) (AA 29-248) protein (His tag)BTN2A1 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
butyrophilin, Subfamily 2, Member A1 (BTN2A1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)BTN2A1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 52.7 kDa. The protein migrates as 65-70 kDa under reducing (R) condition due to glycosylation.
- 说明
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- BTN2A1 (butyrophilin, Subfamily 2, Member A1 (BTN2A1))
- 别名
- BTN2A1 (BTN2A1 产品)
- 别名
- BK14H9.1 Protein, BT2.1 Protein, BTF1 Protein, DJ3E1.1 Protein, BTN2A1 Protein, btf1 Protein, bt2.1 Protein, BTN2A2 Protein, butyrophilin subfamily 2 member A1 Protein, butyrophilin subfamily 2 member A1 S homeolog Protein, butyrophilin, subfamily 2, member A1 Protein, BTN2A1 Protein, btn2a1.S Protein, btn2a1 Protein, LOC100409586 Protein
- 背景
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Synonyms: BK14H9.1,BT2.1,BTF1,BTF1BT2.1butyrophilin BTF1,BTN2A1,butyrophilin subfamily 2 member A1,butyrophilin,subfamily 2,member A1,DJ3E1.1,FLJ36567,
Description: Duplication events have led to three paralogues of BTN2A in primates: BTN2A1, BTN2A2, and BTN2A3. In humans, only BTN2A1 has been functionally characterised, it has been detected on epithelial cells and leukocytes, and identified as a novel ligand of dendritic cell-specific ICAM-3 grabbing nonintegrin (DCSIGN), a C-type lectin receptor that acts as an internalization receptor for HIV-1, HCV, and other pathogens. BTN2A2 mRNA has been shown to be expressed in circulating human immune cells. - 分子量
- 52.7 kDa
- 途径
- Activated T Cell Proliferation
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