Sonic Hedgehog Protein (SHH) (His tag)
Quick Overview for Sonic Hedgehog Protein (SHH) (His tag) (ABIN7455651)
抗原
See all Sonic Hedgehog (SHH) 蛋白蛋白类型
宿主
资源
纯度
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标记
- This Sonic Hedgehog protein is labelled with His tag.
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原理
- Recombinant Human Sonic Hedgehog is produced by our Mammalian expression system and the target gene encoding Cys24-Gly197 is expressed with a 6His tag at the C-terminus.
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产品特性
- Extracellular Domain Protein
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纯化方法
- Purified from cell culture supernatant by affinity chromatography
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Sonic Hedgehog (SHH) (AA 25-198), (Cys25IleIle-Mutant) (Active) protein
SHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 24-197), (Cys24IleIle-Mutant) (Active) proteinSHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 24-197) proteinSHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sonic Hedgehog (SHH) protein (His tag)SHH 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) proteinSHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) (Active) protein (His tag)SHH 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE SDS
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Sonic Hedgehog (SHH) proteinSHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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有效期
- 12 months
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- Sonic Hedgehog (SHH)
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别名
- SHH
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背景
- Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45 kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the ""all signaling"" capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc.
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分子量
- 20.4 KDa
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UniProt
- Q15465
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途径
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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