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MICB Protein (AA 23-298) (Fc Tag)

MICB 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7454188
发货至: 中国
  • 抗原 See all MICB 蛋白
    MICB (MHC Class I Polypeptide-Related Sequence B (MICB))
    蛋白类型
    Recombinant
    产品特性
    AA 23-298
    宿主
    资源
    • 9
    • 3
    • 2
    • 2
    HEK-293 Cells
    标记
    This MICB protein is labelled with Fc Tag.
    原理
    Human MICB Protein
    序列
    Ala23-Gly298
    产品特性
    Recombinant Human MICB Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala23-Gly298.
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human MICB, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-MICB Antibody, hFc Tag with the EC50 of 54.9ng/ml determined by ELISA (QC Test). The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
    Top Product
    Discover our top product MICB 蛋白
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    MICB (MHC Class I Polypeptide-Related Sequence B (MICB))
    别名
    MICB (MICB 产品)
    别名
    PERB11.2 Protein, MHC class I polypeptide-related sequence B Protein, MHC class I-related protein-like Protein, MICB Protein, LOC512825 Protein
    背景
    MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85 % amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
    分子量
    58.21 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
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