CLEC4C
(C-Type Lectin Domain Family 4, Member C (CLEC4C))
蛋白类型
Recombinant
产品特性
AA 46-213
宿主
人
资源
HEK-293 Cells
标记
This CLEC4C protein is labelled with His tag.
原理
Human BDCA-2 Protein
序列
Phe46-Ile213
产品特性
Recombinant Human BDCA-2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Phe46-Ile213.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human BDCA-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). The affinity constant of 2.97 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Supplied as 0.22μm filtered solution in 20 mM Tris, 300 mM NaCl, 200 mM L-arginine ( pH 8.5).
储存条件
-80 °C
储存方法
Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
CLEC4C
(C-Type Lectin Domain Family 4, Member C (CLEC4C))
BDCA2 Protein, CD303 Protein, CLECSF11 Protein, CLECSF7 Protein, DLEC Protein, HECL Protein, PRO34150 Protein, Clec4c Protein, C-type lectin domain family 4 member C Protein, CLEC4C Protein, LOC100733227 Protein
背景
BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.
分子量
20.93 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.