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SPARC Protein (AA 18-303) (His tag)

SPARC 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7275639
发货至: 中国
  • 抗原 See all SPARC 蛋白
    SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
    蛋白类型
    Recombinant
    产品特性
    AA 18-303
    宿主
    • 14
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Cynomolgus
    资源
    • 10
    • 7
    • 5
    • 2
    • 1
    • 1
    HEK-293 Cells
    标记
    This SPARC protein is labelled with His tag.
    原理
    Cynomolgus SPARC Protein
    序列
    Ala18-Ile303
    产品特性
    Recombinant Cynomolgus SPARC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala18-Ile303.
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Cynomolgus SPARC, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA. See testing image for detail.
    Top Product
    Discover our top product SPARC 蛋白
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
    别名
    SPARC (SPARC 产品)
    别名
    ON Protein, AA517111 Protein, BM-40 Protein, hm:zeh0062 Protein, SPARC Protein, sparc Protein, secreted protein acidic and cysteine rich Protein, secreted acidic cysteine rich glycoprotein Protein, secreted protein, acidic, cysteine-rich (osteonectin) Protein, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog Protein, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog Protein, SPARC Protein, Sparc Protein, sparc Protein, sparc.L Protein, sparc.S Protein
    背景
    Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.
    分子量
    33.82 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.
    UniProt
    G7P8R2
    途径
    Autophagy
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