MAG Protein (AA 20-516) (His-Avi Tag,Biotin)
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- 抗原 See all MAG 蛋白
- MAG (Myelin Associated Glycoprotein (MAG))
- 蛋白类型
- Recombinant
- 产品特性
- AA 20-516
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This MAG protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Human Siglec-4a/MAG Protein
- 序列
- Gly20-Pro516
- 产品特性
- Recombinant Biotinylated Human Siglec-4a/MAG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly20-Pro516.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-Siglec-4a Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 21.5ng/ml determined by ELISA. See testing image for detail.
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Myelin Associated Glycoprotein (MAG) (AA 20-516) protein (His tag)
MAG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Myelin Associated Glycoprotein (MAG) (AA 20-516) protein (His tag)MAG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Myelin Associated Glycoprotein (MAG) (AA 20-516) protein (Fc Tag)MAG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Myelin Associated Glycoprotein (MAG) (AA 1-626) protein (GST tag)MAG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Myelin Associated Glycoprotein (MAG) (AA 119-208) protein (GST tag)MAG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Myelin Associated Glycoprotein (MAG) protein (Fc Tag,ECD)MAG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Myelin Associated Glycoprotein (MAG) (AA 20-516) protein (His tag)Crystallography grade MAG 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Myelin Associated Glycoprotein (MAG) (AA 20-516) protein (His tag)Crystallography grade MAG 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Myelin Associated Glycoprotein (MAG) (AA 20-626) protein (rho-1D4 tag)Crystallography grade MAG 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Myelin Associated Glycoprotein (MAG) (AA 20-626) protein (rho-1D4 tag)Crystallography grade MAG 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- MAG (Myelin Associated Glycoprotein (MAG))
- 别名
- Siglec-4a (MAG 产品)
- 别名
- GMA Protein, S-MAG Protein, SIGLEC-4A Protein, SIGLEC4A Protein, siglec-4 Protein, MAG Protein, Gma Protein, siglec-4a Protein, myelin associated glycoprotein Protein, myelin-associated glycoprotein Protein, MAG Protein, mag Protein, Mag Protein
- 背景
- Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity).
- 分子量
- 57.6 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Tris-Bis PAGE result.
- UniProt
- P20916
- 途径
- Neurotrophin Signaling Pathway
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