SIGLEC10 Protein (AA 17-546) (His-Avi Tag,Biotin)
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- 抗原 See all SIGLEC10 蛋白
- SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))
- 蛋白类型
- Recombinant
- 产品特性
- AA 17-546
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This SIGLEC10 protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Human Siglec-10 Protein
- 序列
- Met17-Thr546
- 产品特性
- Recombinant Biotinylated Human Siglec-10 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Thr546.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-Siglec-10, hFc Tag Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-10, His Tag with the EC50 of 92.9ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product SIGLEC10 蛋白
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Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (AA 17-546) protein (His tag)
SIGLEC10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) protein (Fc-Avi Tag,Biotin)SIGLEC10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (Arg119Ala-Mutant) protein (His-Avi Tag,Biotin)SIGLEC10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (AA 17-546) protein (Fc-Avi Tag)SIGLEC10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (Arg119Ala-Mutant) protein (His-Avi Tag)SIGLEC10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (AA 17-552) protein (His tag)SIGLEC10 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (Active) protein (mFc Tag)SIGLEC10 宿主: 人 宿主: 人细胞 Recombinant >90 % as determined by reducing SDS-PAGE. Active
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (Active) protein (Fc Tag)SIGLEC10 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) (Active) protein (Biotin)SIGLEC10 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10) protein (Fc Tag)SIGLEC10 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in 20 mM Tris, 150 mM Nacl, 200 mM Arginine ( pH 8.5). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))
- 别名
- Siglec-10 (SIGLEC10 产品)
- 别名
- PRO940 Protein, SIGLEC-10 Protein, SLG2 Protein, Siglecg Protein, SIGLEC10 Protein, slg2 Protein, pro940 Protein, siglec-10 Protein, sialic acid binding Ig like lectin 10 Protein, sialic acid binding Ig-like lectin 10 Protein, sialic acid-binding Ig-like lectin 10 Protein, SIGLEC10 Protein, Siglec10 Protein, LOC484343 Protein, LOC100066644 Protein, siglec10 Protein, LOC100513863 Protein, LOC100340374 Protein
- 背景
- Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
- 分子量
- 61.13 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
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