CD22 Protein (AA 20-687) (Fc Tag)
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- 抗原 See all CD22 蛋白
- CD22 (CD22 Molecule (CD22))
- 蛋白类型
- Recombinant
- 产品特性
- AA 20-687
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CD22 protein is labelled with Fc Tag.
- 原理
- Human Siglec-2/CD22 Protein
- 序列
- Asp20-Arg687
- 产品特性
- Recombinant Human Siglec-2/CD22 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp20-Arg687.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Siglec-2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 46.6ng/ml determined by ELISA. See testing image for detail.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD22 (CD22 Molecule (CD22))
- 别名
- Siglec2 (CD22 产品)
- 别名
- A530093D23 Protein, Lyb-8 Protein, Lyb8 Protein, SIGLEC-2 Protein, SIGLEC2 Protein, siglec-2 Protein, CD22 antigen Protein, CD22 molecule Protein, Cd22 Protein, CD22 Protein
- 背景
- CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice.
- 分子量
- 101.9 kDa. Due to glycosylation, the protein migrates to 120-130 kDa based on Tris-Bis PAGE result.
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