Sonic Hedgehog Protein (SHH) (AA 24-197)
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Quick Overview for Sonic Hedgehog Protein (SHH) (AA 24-197) (ABIN7275581)
抗原
See all Sonic Hedgehog (SHH) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 24-197
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原理
- Human Sonic Hedgehog (Shh) Protein
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序列
- Cys24-Gly197
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产品特性
- Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag.It contains Cys24-Gly197.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 0.05 EU per μg by the LAL method.
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Biological Activity Comment
- Human Sonic Hedgehog (Shh) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50-for this effect is 0.1-0.4 μg/mL.,The purity of Human Sonic Hedgehog (Shh) is greater than 95% as determined by SEC-HPLC.
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Sonic Hedgehog (SHH) (AA 25-198), (Cys25IleIle-Mutant) (Active) protein
SHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 24-197), (Cys24IleIle-Mutant) (Active) proteinSHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) protein (His tag)SHH 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) proteinSHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) protein (His tag)SHH 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) protein (His tag)SHH 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
Sonic Hedgehog (SHH) proteinSHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS, 300 mM NaCl ( pH 7.4). Normally 8 % trehalose / 8 % mannitol is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Sonic Hedgehog (SHH)
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别名
- Sonic Hedgehog
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背景
- Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages.
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分子量
- 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
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UniProt
- Q15465
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途径
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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