IL12B Protein (AA 23-328) (His tag)
-
- 抗原 See all IL12B 蛋白
- IL12B (Interleukin 12 beta (IL12B))
- 蛋白类型
- Recombinant
- 产品特性
- AA 23-328
-
宿主
- Cynomolgus
-
资源
- HEK-293 Cells
- 标记
- This IL12B protein is labelled with His tag.
- 原理
- Cynomolgus IL-12B/p40/NKSF2 Protein
- 序列
- Ile23-Ser328
- 产品特性
- Recombinant Cynomolgus IL-12B/p40/NKSF2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Ser328.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus IL-12B, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product IL12B 蛋白
-
Interleukin 12 beta (IL12B) (AA 23-328) protein (His tag)
IL12B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 12 beta (IL12B) (AA 23-328) protein (His tag)IL12B 宿主: 人 宿主: Tobacco (Nicotiana benthamiana) Recombinant > 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. WB, Imm
Interleukin 12 beta (IL12B) (AA 23-328) proteinIL12B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Interleukin 12 beta (IL12B) protein (His tag)IL12B 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 12 beta (IL12B) (AA 229-328) protein (GST tag)IL12B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 12 beta (IL12B) (AA 23-328) protein (His tag)IL12B 宿主: 人 宿主: Insect Cells Recombinant SDS
Interleukin 12 beta (IL12B) (AA 23-335) protein (His tag)Crystallography grade IL12B 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 12 beta (IL12B) (AA 23-328) protein (Fc Tag)IL12B 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Interleukin 12 beta (IL12B) (AA 23-328) (Active) protein (His tag)IL12B 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 12 beta (IL12B) (AA 23-328), (full length) protein (GST tag)IL12B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- IL12B (Interleukin 12 beta (IL12B))
- 别名
- IL-12B (IL12B 产品)
- 别名
- CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, IL-12 p40 Protein, il12b Protein, il12p40.b Protein, zgc:152789 Protein, IL-12.p40 Protein, IL12p40 Protein, IL12B Protein, CLMF p40 Protein, interleukin 12B Protein, interleukin 12b Protein, interleukin 12Ba Protein, IL12B Protein, Il12b Protein, il12ba Protein
- 背景
- Interleukin (IL)-12B, which encodes the p40 subunit common to IL-12 and IL-23, as one of the genes for which expression in fibroblast-like synoviocytes from patients with rheumatoid arthritis (RA-FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin-related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor-like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand.
- 分子量
- 35.86 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
- UniProt
- A0A2K5TM36
- 途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
-
