NOTCH3 Protein (AA 1378-1640) (His tag)
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- 抗原 See all NOTCH3 蛋白
- NOTCH3 (Notch 3 (NOTCH3))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1378-1640
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This NOTCH3 protein is labelled with His tag.
- 原理
- Human Notch 3 Protein
- 序列
- Ala1378-Ser1640
- 产品特性
- Recombinant Human Notch 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala1378-Ser1640.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Notch 3, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail.
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Notch 3 (NOTCH3) protein (His-Avi Tag)
NOTCH3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Notch 3 (NOTCH3) (AA 40-467) protein (Fc Tag)NOTCH3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Notch 3 (NOTCH3) (AA 47-156) protein (GST tag)NOTCH3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Notch 3 (NOTCH3) (AA 1665-2321) protein (His tag)Crystallography grade NOTCH3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Notch 3 (NOTCH3) (AA 1665-2318) protein (His tag)Crystallography grade NOTCH3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Notch 3 (NOTCH3) (AA 40-1643) protein (His tag)Crystallography grade NOTCH3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Notch 3 (NOTCH3) (AA 40-1643) protein (His tag)Crystallography grade NOTCH3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Notch 3 (NOTCH3) (AA 1629-2321) protein (rho-1D4 tag)Crystallography grade NOTCH3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Notch 3 (NOTCH3) (AA 1662-2321) protein (rho-1D4 tag)Crystallography grade NOTCH3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Notch 3 (NOTCH3) (AA 1630-2318) protein (rho-1D4 tag)Crystallography grade NOTCH3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- NOTCH3 (Notch 3 (NOTCH3))
- 别名
- Notch 3 (NOTCH3 产品)
- 别名
- CADASIL Protein, CASIL Protein, IMF2 Protein, AW229011 Protein, N3 Protein, hpbk Protein, fa14b08 Protein, notch5 Protein, wu:fa14b08 Protein, notch 3 Protein, NOTCH3 Protein, Notch3 Protein, notch3 Protein
- 背景
- Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
- 分子量
- 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9UM47
- 途径
- Notch Signaling
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