KLRK1
(Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))
蛋白类型
Recombinant
产品特性
AA 73-216
宿主
Cynomolgus
资源
HEK-293 Cells
标记
This KLRK1 protein is labelled with His tag.
原理
Cynomolgus NKG2D/CD314 Protein
序列
Ile73-Val216
产品特性
Recombinant Cynomolgus NKG2D/CD314 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ile73-Val216.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus NKG2D, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
KLRK1
宿主: 小鼠
宿主: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
KLRK1
(Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
分子量
17.48 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.