PVRL2
(Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))
蛋白类型
Recombinant
产品特性
AA 32-351
宿主
小鼠
资源
HEK-293 Cells
标记
This PVRL2 protein is labelled with His tag.
原理
Mouse Nectin-2/CD112 Protein
序列
Gln32-Gly351
产品特性
Recombinant Mouse Nectin-2/CD112 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Gly351.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Mouse PVRIG, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse Nectin-2, His Tag with the EC50 of 0.65ug/ml determined by ELISA. The affinity constant of 0.355 μM as determined in SPR assay (Biacore T200). See testing image for detail.
PVRL2
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 98 % as determined by SDS-PAGE
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
PVRL2
(Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))
Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
分子量
35.7 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.