PVRL2
(Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
标记
This PVRL2 protein is labelled with His-Avi Tag,Biotin.
序列
Gln32-Leu360
纯度
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human Nectin-2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 2.26μg/ml determined by ELISA. See testing image for detail.
PVRL2
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 98 % as determined by SDS-PAGE
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose and 0.01 % tween is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
有效期
12 months
抗原
PVRL2
(Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))
CD112, MPH, Nectin2, PRR2, PVRL2, HVEB, PVRR2,Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
分子量
38.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.