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nectin-3 Protein (AA 58-400) (His-Avi Tag)

NECTIN3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7275311
发货至: 中国
  • 抗原 See all nectin-3 (NECTIN3) 蛋白
    nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))
    蛋白类型
    Recombinant
    产品特性
    AA 58-400
    宿主
    • 17
    • 4
    资源
    • 7
    • 4
    • 4
    • 3
    • 2
    • 1
    HEK-293 Cells
    标记
    This nectin-3 protein is labelled with His-Avi Tag.
    原理
    Human Nectin-3/CD113 Protein
    序列
    Gly58-Asp400
    产品特性
    Recombinant Human Nectin-3/CD113 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly58-Asp400.
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    The affinity constant of 44μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in 20 mM PB, 250 mM NaCl ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))
    别名
    Nectin-3 (NECTIN3 产品)
    别名
    PVRL3 Protein, CD113 Protein, CDW113 Protein, NECTIN-3 Protein, PPR3 Protein, PRR3 Protein, PVRR3 Protein, 2610301B19Rik Protein, 3000002N23Rik Protein, 4921513D19Rik Protein, AA407785 Protein, AU016832 Protein, AW538082 Protein, nectin cell adhesion molecule 3 Protein, poliovirus receptor-related 3 Protein, Nectin3 Protein, NECTIN3 Protein, PVRL3 Protein, nectin3 Protein
    背景
    The Nectin family has at least four members (Nectin-1-4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids.
    分子量
    40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on Tris-Bis PAGE result.
    UniProt
    Q9NQS3
    途径
    Cell-Cell Junction Organization
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