LILRB1
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 1 (LILRB1))
蛋白类型
Recombinant
产品特性
AA 24-221
宿主
人
资源
HEK-293 Cells
标记
This LILRB1 protein is labelled with His tag.
原理
Human LILRB1/CD85j/ILT2 Domain1 & 2 Protein
序列
Gly24-Gly221
产品特性
Recombinant Human LILRB1/CD85j/IL-T2 Domain1 & 2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Gly221.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human LILRB1 Domain1&2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA. The affinity constant of 1.9μM as determined in SPR assay (Biacore T200). See testing image for detail.
LILRB1
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
LILRB1
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 1 (LILRB1))
LILRB1, also known as CD85j and IL-T2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens.
分子量
23.1 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.