LILRB1 Protein (AA 24-458) (Biotin,His-Avi Tag)
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Quick Overview for LILRB1 Protein (AA 24-458) (Biotin,His-Avi Tag) (ABIN7275186)
抗原
See all LILRB1 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 24-458
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标记
- This LILRB1 protein is labelled with Biotin,His-Avi Tag.
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原理
- Biotinylated Human LILRB1/CD85j/ILT2 Protein
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序列
- Gly24-His458
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产品特性
- Recombinant Biotinylated Human LILRB1/CD85j/ILT2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-His458.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Biotinylated Human LILRB1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA (QC Test).,Biotinylated Human LILRB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of-Biotinylated Human LILRB1 is greater than 95% as determined by SEC-HPLC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- LILRB1 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 1 (LILRB1))
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别名
- LILRB1
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背景
- LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response.
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分子量
- 50 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
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UniProt
- Q8NHL6
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
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