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LIFR Protein (AA 45-833) (His tag)

LIFR 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7275152
发货至: 中国
  • 抗原 See all LIFR 蛋白
    LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))
    蛋白类型
    Recombinant
    产品特性
    AA 45-833
    宿主
    • 13
    • 9
    • 5
    资源
    • 9
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    HEK-293 Cells
    标记
    This LIFR protein is labelled with His tag.
    原理
    Human LIF R/CD118 Protein
    序列
    Gln45-Ser833
    产品特性
    Recombinant Human LIF R/CD118 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln45-Ser833.
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human LIF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.75μg/ml determined by ELISA. The affinity constant of 0.662 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))
    别名
    LIF R (LIFR 产品)
    别名
    A230075M04Rik Protein, AW061234 Protein, LIF Protein, CD118 Protein, LIF-R Protein, SJS2 Protein, STWS Protein, SWS Protein, leukemia inhibitory factor receptor Protein, LIF receptor alpha Protein, leukemia inhibitory factor receptor alpha Protein, Lifr Protein, LOC397451 Protein, LIFR Protein
    背景
    The leukemia inhibitory factor receptor (LIF-R) subunit is a component of cell-surface receptor complexes for the multifunctional cytokines, LIF, cardiotrophin-1, ciliary neurotrophic factor, and human oncostatin M. The structure of the human LIF-R gene is similar to that of the mouse gene. The transmembrane receptor is encoded by 19 exons.
    分子量
    90.5 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
    途径
    JAK/STAT Signaling, Growth Factor Binding
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