IL-2 R beta & IL-2 R gamma (AA 27-240) protein (Fc Tag)
-
- 抗原
- IL-2 R beta & IL-2 R gamma
- 蛋白类型
- Recombinant
- 产品特性
- AA 27-240
-
宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- Fc Tag
- 原理
- Human IL-2 R beta & IL-2 R gamma Protein
- 序列
- Ala27-Thr240 (IL-2 R beta) & Leu23-Ala262 (IL-2 R gamma)
- 特异性
- Uni-Prot: P14784 (IL-2 R beta), P31785-1 (IL-2 R gamma)
- 产品特性
- Recombinant Human IL-2 R beta & IL-2 R gamma Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala27-Thr240 (IL-2 R beta) & Leu23-Ala262 (IL-2 R gamma).
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-15RA&IL-15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA. The affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). See testing image for detail.
-
IL-2 R beta & IL-2 R gamma (AA 27-239) (Active) protein (Fc Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
IL-2 R beta & IL-2 R gamma (AA 27-239) (Active) protein (Fc Tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
IL-2 R beta & IL-2 R gamma (AA 27-239) (Active) protein (His tag,Strep Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- IL-2 R beta & IL-2 R gamma
- 背景
- Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change.
- 分子量
- 51.3 kDa (IL-2 R beta)&54.6 kDa (IL-2 R gamma). Due to glycosylation, the protein migrates to 65-70 kDa&80-100 kDa based on Tris-Bis PAGE result.
- UniProt
- P14784
-
