IL-2 R beta & IL-2 R gamma protein (Fc-Avi Tag,Biotin)
-
- 抗原
- IL-2 R beta & IL-2 R gamma
- 蛋白类型
- Recombinant
-
宿主
- Cynomolgus
-
资源
- HEK-293 Cells
- 标记
- Fc-Avi Tag,Biotin
- 原理
- Biotinylated Cynomolgus IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc,Avitag™&Fc,Avitag™ (MALS verified)
- 序列
- Ala 27 - Thr 240 (IL-2RB) & Leu 23 - Pro 255 (IL-2RG)
- 产品特性
- Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag (ILG-C82F4) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Thr 240 (IL-2RB) & Leu 23 - Pro 255 (IL-2RG) (Accession # Q38J85-1 (IL-2RB) & Q38JL2-1(IL-2RG).
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- 质量等级
- MALS verified
-
IL-2 R beta & IL-2 R gamma (AA 27-240) protein (Fc Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IL-2 R beta & IL-2 R gamma (AA 27-239) (Active) protein (Fc Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
IL-2 R beta & IL-2 R gamma (AA 27-239) (Active) protein (Fc Tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
IL-2 R beta & IL-2 R gamma (AA 27-239) (Active) protein (His tag,Strep Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
-
- 应用备注
- Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB andIL-2RG, has a calculated MW of 53.0 kDa (IL-2RB) and 55.4 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™) and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The reducing (R) protein migrates as 70-85 kDa and 90-110 kDa respectively due to glycosylation.
- 说明
-
Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 储存条件
- -20 °C
- 储存方法
- -20°C
-
- 抗原
- IL-2 R beta & IL-2 R gamma
- 别名
- IL-2 R beta&IL-2 R gamma
- 背景
-
Synonyms: IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG,
Description: Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. - 分子量
- 53.0 kDa & 55.4 kDa
-
