Recombinant Human IL-17A & F Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F).
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA. See testing image for detail.
IL-17A/F
宿主: 人
宿主: 人细胞
Recombinant
Greater than 95 % as determined by reducing SDS-PAGE.
ELISA, MS, SDS, WB
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
il17-5 Protein, si:ch211-63o20.2 Protein, interleukin 17a/f3 Protein, il17a/f3 Protein
背景
Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
分子量
15.1 kDa (IL-17A) & 15.7 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa & 27 kDa based on Tris-Bis PAGE result.