IL-5 Protein (AA 20-134) (His-Avi Tag)
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- 抗原 See all IL-5 (IL5) 蛋白
- IL-5 (IL5) (Interleukin 5 (IL5))
- 蛋白类型
- Recombinant
- 产品特性
- AA 20-134
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This IL-5 protein is labelled with His-Avi Tag.
- 原理
- Human IL-5 Protein
- 序列
- Ile20-Ser134
- 产品特性
- Recombinant Human IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ile20-Ser134.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-5 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 47.7ng/ml determined by ELISA. The affinity constant of 4.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 5 (IL5) (AA 20-134) protein (His-Avi Tag,Biotin)
IL5 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 21-133) protein (His-Avi Tag)IL5 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 20-134) protein (His tag)IL5 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 21-133) protein (His-Avi Tag,Biotin)IL5 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 (IL5) (AA 20-132) protein (His tag)IL5 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 5 (IL5) (AA 21-133) protein (His tag)IL5 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 5 (IL5) protein (His tag)IL5 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 5 (IL5) (AA 1-134) protein (GST tag)IL5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 5 (IL5) (AA 20-134) protein (GST tag)IL5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 5 (IL5) protein (His tag)IL5 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- IL-5 (IL5) (Interleukin 5 (IL5))
- 别名
- IL-5 (IL5 产品)
- 别名
- EDF Protein, IL-5 Protein, TRF Protein, Il-5 Protein, IL5 Protein, interleukin 5 Protein, IL5 Protein, Il5 Protein
- 背景
- IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).
- 分子量
- 16.1 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.
- UniProt
- P05113
- 途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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