IL-31 Protein (AA 24-164) (His tag)
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- 抗原 See all IL-31 (IL31) 蛋白
- IL-31 (IL31) (Interleukin 31 (IL31))
- 蛋白类型
- Recombinant
- 产品特性
- AA 24-164
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This IL-31 protein is labelled with His tag.
- 原理
- Human IL-31 Protein
- 序列
- Ser24-Thr164
- 产品特性
- Recombinant Human IL-31 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ser24-Thr164.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-31, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.2μg/ml determined by ELISA. The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- IL-31 (IL31) (Interleukin 31 (IL31))
- 别名
- IL-31 (IL31 产品)
- 别名
- IL-31 Protein, 1700013B14Rik Protein, IL31 Protein, interleukin 31 Protein, IL31 Protein, Il31 Protein
- 背景
- Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells.
- 分子量
- 16.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
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