This IL-3 protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Human IL-3 Protein
序列
Ala20-Phe152
产品特性
Recombinant Biotinylated Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Ala20-Phe152.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human IL-3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 1.3μg/ml determined by ELISA. See testing image for detail.
IL-3
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % as analyzed by SDS-PAGE and HPLC.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.