IL-10RA Protein (AA 22-233) (His tag)
IL10RA
宿主: Cynomolgus
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- 抗原 See all IL-10RA (IL10RA) 蛋白
- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
- 蛋白类型
- Recombinant
- 产品特性
- AA 22-233
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宿主
- Cynomolgus
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资源
- HEK-293 Cells
- 标记
- This IL-10RA protein is labelled with His tag.
- 原理
- Cynomolgus IL-10 R alpha Protein
- 序列
- His22-Asn233
- 产品特性
- Recombinant Cynomolgus IL-10 R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His22-Asn233.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-10, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus IL-10 R alpha, His Tag with the EC50 of 8.9ng/ml determined by ELISA. The affinity constant of 0.93 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Discover our top product IL10RA 蛋白
-
Interleukin 10 Receptor, alpha (IL10RA) (AA 22-235) protein (His-Avi Tag)
IL10RA 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
- 别名
- IL-10 R alpha (IL10RA 产品)
- 别名
- IL10R1 Protein, IL10RA Protein, CD210 Protein, CD210a Protein, CDW210A Protein, HIL-10R Protein, IL-10R1 Protein, IL10R Protein, AW553859 Protein, CDw210 Protein, CDw210a Protein, Il10r Protein, mIL-10R Protein, interleukin 10 receptor subunit alpha Protein, interleukin 10 receptor, alpha Protein, IL10RA Protein, Il10ra Protein
- 背景
- The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.
- 分子量
- 25.34 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
- UniProt
- A0A2K5WJ66
- 途径
- Growth Factor Binding
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