HLAG Protein (Tetramer) (HLA-G)
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- 抗原 See all HLAG 蛋白
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
- 蛋白类型
- Recombinant
- 产品特性
- Tetramer
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This HLAG protein is labelled with HLA-G.
- 原理
- Human HLA-G & B2M & Peptide (RIIPRHLQL) Tetramer Protein
- 序列
- Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide
- 特异性
- Uni-Prot: P17693-1 (HLA-G), P61769 (B2M), RIIPRHLQL
- 产品特性
- Recombinant Human HLA-G & B2M & Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 4.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Tetramer) protein (HLA-G)
HLAG 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Tetramer) protein (Biotin,HLA-G)HLAG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Monomer) protein (Biotin,HLA-G)HLAG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Tetramer) protein (HLA-G)HLAG 宿主: 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Monomer) protein (HLA-G)HLAG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Monomer) protein (HLA-G)HLAG 宿主: 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Monomer) protein (HLA-G)HLAG 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Tetramer) protein (PE,HLA-G)HLAG 宿主: 人 宿主: HEK-293 Cells Recombinant
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (AA 1-338) protein (GST tag)HLAG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (AA 25-308) protein (His tag)Crystallography grade HLAG 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
- 别名
- HLA-G (HLAG 产品)
- 别名
- MHC-G Protein, B-F Protein, B-F-S04 Protein, B-F-S05 Protein, B-F-S06 Protein, B-F-S07 Protein, B-FI Protein, B-FIV Protein, BF2 Protein, BFa2 Protein, BFw-03 Protein, BFw-05 Protein, BFz-01 Protein, major histocompatibility complex, class I, G Protein, MHC BF1 class I Protein, HLA-G Protein, BF1 Protein
- 背景
- HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
- 分子量
- 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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