GFRA3 Protein (AA 32-382) (His tag)
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- 抗原 See all GFRA3 蛋白
- GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))
- 蛋白类型
- Recombinant
- 产品特性
- AA 32-382
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This GFRA3 protein is labelled with His tag.
- 原理
- Human GFRA3 Protein
- 序列
- Asp32-Trp382
- 产品特性
- Recombinant Human GFRA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp32-Trp382.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GFRA3, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-GFRA3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA. See testing image for detail.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))
- 别名
- GFRA3 (GFRA3 产品)
- 别名
- GFRA3 Protein, GFRalpha3 Protein, Y15110 Protein, GDNF family receptor alpha 3 Protein, glial cell line derived neurotrophic factor family receptor alpha 3 Protein, Gfra3 Protein, GFRA3 Protein
- 背景
- GDNF (glial-cell-line derived neurotrophic factor) is a potent neurotrophic factor for dopaminergic neurons. Neuropsychiatric diseases and their treatments are associated with alterations in the levels of both GDNF and its receptor family (GDNF family receptor alpha or GFRA). GFRA1, GFRA2 and GFRA3 are located in chromosomal regions with suggestive linkage to schizophrenia.
- 分子量
- 40.3 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.
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