GFRAL Protein (His-Avi Tag)
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- 抗原 See all GFRAL 蛋白
- GFRAL (GDNF Family Receptor alpha Like (GFRAL))
- 蛋白类型
- Recombinant
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This GFRAL protein is labelled with His-Avi Tag.
- 序列
- Gln20-Glu350
- 纯度
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Mouse GFRAL, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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GDNF Family Receptor alpha Like (GFRAL) (AA 19-351) protein (His-Avi Tag)
GFRAL 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha Like (GFRAL) (AA 19-351) protein (His-Avi Tag,Biotin)GFRAL 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha Like (GFRAL) (AA 19-351) protein (Fc Tag)GFRAL 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha Like (GFRAL) (AA 20-351) protein (His-Avi Tag)GFRAL 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha Like (GFRAL) (AA 20-350) protein (His-Avi Tag,Biotin)GFRAL 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha Like (GFRAL) (AA 20-351) protein (His-Avi Tag,Biotin)GFRAL 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha Like (GFRAL) (AA 20-349) protein (His tag)Crystallography grade GFRAL 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
GDNF Family Receptor alpha Like (GFRAL) (AA 19-351) protein (His tag)Crystallography grade GFRAL 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
GDNF Family Receptor alpha Like (GFRAL) (AA 19-394) protein (rho-1D4 tag)Crystallography grade GFRAL 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
GDNF Family Receptor alpha Like (GFRAL) (AA 20-393) protein (rho-1D4 tag)Crystallography grade GFRAL 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- GFRAL (GDNF Family Receptor alpha Like (GFRAL))
- 别名
- GFRAL (GFRAL 产品)
- 别名
- RGD1565220 Protein, C6orf144 Protein, GRAL Protein, UNQ9356 Protein, bA360D14.1 Protein, AY457637 Protein, Gral Protein, GDNF family receptor alpha like Protein, Gfral Protein, GFRAL Protein
- 背景
- GFR alpha-like, GFRAL, GRAL, C6orf144,GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
- 分子量
- 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
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