This GUCY2C protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Human GUCY2C/Guanylyl cyclase C Protein
序列
Ser24-Gln430
产品特性
Recombinant Biotinylated Human GUCY2C/Guanylyl cyclase C Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser24-Gln430.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-GUCY2C Antibody at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GUCY2C, His Tag with the EC50 of 24.1ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
GUCY2C
宿主: 小鼠
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, Crys, SDS
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Guanylyl cyclase C (GUCY2C) has canonical centrality in defense of key intestinal homeostatic mechanisms, encompassing fluid and electrolyte balance, epithelial dynamics, antitumorigenesis, and intestinal barrier function. GUCY2C may represent a new target for anti-obesity pharmacotherapy.
分子量
48.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.