This LGALS1/Galectin 1 protein is labelled with Fc Tag.
原理
Human Galectin 1 Protein
序列
Ala2-Asp135
产品特性
Recombinant Human Galectin 1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala2-Asp135.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human GAL1, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-GAL1 Antibody, hFc Tag with the EC50 of 40.6ng/ml determined by ELISA. See testing image for detail.
LGALS1
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % by SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Galectin 1(Gal-1), a β-galactoside binding mammalian lectin of 14KDa, is implicated in many signalling pathways, immune responses associated with cancer progression and immune disorders. Inhibition of human Gal-1 has been regarded as one of the potential therapeutic approaches for the treatment of cancer, as it plays a major role in tumour development and metastasis by modulating various biological functions viz. apoptosis, angiogenesis, migration, cell immune escape.
分子量
41.9 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.