LGALS1/Galectin 1 Protein (AA 2-135) (His tag)
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- 抗原 See all LGALS1/Galectin 1 (LGALS1) 蛋白
- LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-135
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宿主
- 人
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资源
- Insect Cells
- 标记
- This LGALS1/Galectin 1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
- MHHHHHHACG LVASNLNLKP GECLRVRGEV APDAKSFVLN LGKDSNNLCL HFNPRFNAHG DANTIVCNSK DGGAWGTEQR EAVFPFQPGS VAEVCITFDQ ANLTVKLPDG YEFKFPNRLN LEAINYMAAD GDFKIKCVAF D
- 特异性
- N-terminal His-tag
- 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human LGALS1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount..
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product LGALS1 蛋白
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Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) (AA 2-135) protein (Fc Tag)
LGALS1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) (AA 2-135) protein (Fc Tag)LGALS1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) (AA 1-135) protein (GST tag)LGALS1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) (AA 1-100) protein (GST tag)LGALS1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) (AA 2-135) protein (His tag)Crystallography grade LGALS1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 pH 7.4
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
- 别名
- LGALS1 (LGALS1 产品)
- 别名
- GAL1 Protein, GBP Protein, Galectin-1 Protein, LGAS1 Protein, LGALS1 Protein, MGC64502 Protein, GB10026 Protein, AA410090 Protein, Gal-1 Protein, Galbp Protein, L-14.5 Protein, L14 Protein, Lect14 Protein, galectin-1 Protein, Galaptin Protein, galectin 1 Protein, galectin-1 Protein, lectin, galactoside-binding, soluble, 1 L homeolog Protein, protein enabled Protein, lectin, galactose binding, soluble 1 Protein, LGALS1 Protein, Lgals1 Protein, CC1G_05003 Protein, CC1G_05004 Protein, lgals1.L Protein, LOC408848 Protein
- 背景
- Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. {ECO:0000269|PubMed:14617626, ECO:0000269|PubMed:18796645, ECO:0000269|PubMed:19497882, ECO:0000269|PubMed:24945728}.
- 分子量
- 15.5 kDa Including tag.
- UniProt
- P09382
- 途径
- Carbohydrate Homeostasis
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