FGF21 Protein (mFc-Avi Tag)
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- 抗原 See all FGF21 蛋白
- FGF21 (Fibroblast Growth Factor 21 (FGF21))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This FGF21 protein is labelled with mFc-Avi Tag.
- 序列
- His29-Ser209
- 纯度
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human FGF21 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 1.48μg/ml determined by ELISA. The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- FGF21 (Fibroblast Growth Factor 21 (FGF21))
- 别名
- FGF21 (FGF21 产品)
- 别名
- FGF21 Protein, fibroblast growth factor 21 Protein, FGF21 Protein, fgf21 Protein, Fgf21 Protein
- 背景
- UNQ3115, PRO10196, FGF-21, FGF21,Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
- 分子量
- 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
- 途径
- RTK signaling
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