FASL
(Fas Ligand (TNF Superfamily, Member 6) (FASL))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
标记
This FASL protein is labelled with His tag.
序列
Pro134-Leu281
纯度
> 95% as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Fas Ligand, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Crystallography grade
FASL
宿主: 小鼠
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
有效期
12 months
抗原
FASL
(Fas Ligand (TNF Superfamily, Member 6) (FASL))
APTL, CD95-L, Fas ligand, APT1LG1, TNFSF6, FASLG, ALPS1B, CD178, FASL, TNLG1A,Fas ligand (FasL, CD95L) is a 40- kDa type II transmembrane protein that binds to Fas (CD95) receptors and promotes programmed cell death. Fas receptors are expressed at higher levels in many tumors than in normal cells.Somewhat paradoxically, elimination of Fas or FasL from tumors also leads to death induced by CD95 receptor/ligand elimination (DICE).
分子量
18 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.