TNFRSF21
(Tumor Necrosis Factor Receptor Superfamily, Member 21 (TNFRSF21))
蛋白类型
Recombinant
产品特性
AA 42-349
宿主
人
资源
HEK-293 Cells
标记
This TNFRSF21 protein is labelled with His tag.
原理
Human DR6/TNFRSF21 Protein
序列
Gln42-His349
产品特性
Recombinant Human DR6/TNFRSF21 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln42-His349.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human DR6, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DR6 Antibody, hFc Tag with the EC50 of 18.9ng/ml determined by ELISA. See testing image for detail.
TNFRSF21
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by SDS-PAGE
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TNFRSF21
(Tumor Necrosis Factor Receptor Superfamily, Member 21 (TNFRSF21))
Beta-amyloid precursor protein (APP) and death receptor 6 (DR6, also known as TNFRSF21) activate a widespread caspase-dependent self-destruction program. DR6 is broadly expressed by developing neurons, and is required for normal cell body death and axonal pruning both in vivo and after trophic-factor deprivation in vitro.DR6 is activated locally by an inactive surface ligand(s) that is released in an active form after trophic-factor deprivation.
分子量
34.3 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.