DLL4 Protein (AA 27-524) (His-Avi Tag,Biotin)
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- 抗原 See all DLL4 蛋白
- DLL4 (delta-Like 4 (DLL4))
- 蛋白类型
- Recombinant
- 产品特性
- AA 27-524
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This DLL4 protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Human DLL4 Protein
- 序列
- Ser27-Pro524
- 产品特性
- Recombinant Biotinylated Human DLL4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser27-Pro524.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-DLL4 Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human DLL4, His Tag with the EC50 of 93.6ng/ml determined by ELISA. See testing image for detail.
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delta-Like 4 (DLL4) (AA 27-524) protein (Fc Tag)
DLL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
delta-Like 4 (DLL4) (AA 558-685) protein (His-IF2DI Tag)DLL4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
delta-Like 4 (DLL4) (AA 123-221) protein (GST tag)DLL4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
delta-Like 4 (DLL4) (Active) protein (His tag)DLL4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
delta-Like 4 (DLL4) (AA 551-685) protein (His tag)Crystallography grade DLL4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
delta-Like 4 (DLL4) (AA 554-686) protein (His tag)Crystallography grade DLL4 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
delta-Like 4 (DLL4) (AA 27-529) protein (His tag)Crystallography grade DLL4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
delta-Like 4 (DLL4) (AA 27-685) protein (rho-1D4 tag)Crystallography grade DLL4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
delta-Like 4 (DLL4) (AA 27-686) protein (rho-1D4 tag)Crystallography grade DLL4 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- DLL4 (delta-Like 4 (DLL4))
- 别名
- DLL4 (DLL4 产品)
- 别名
- hdelta2 Protein, Delta4 Protein, si:ch211-244p18.5 Protein, zgc:136596 Protein, delta like canonical Notch ligand 4 Protein, delta-like 4 (Drosophila) Protein, DLL4 Protein, Dll4 Protein, dll4 Protein
- 背景
- Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis, negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.
- 分子量
- 55.7 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9NR61
- 途径
- Notch Signaling
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