This CSF2RA protein is labelled with His-Avi Tag,Biotin.
序列
Glu23-Gly320
纯度
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.3ng/ml determined by ELISA. The affinity constant of 1.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Crystallography grade
CSF2RA
宿主: 小鼠
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
GM-CSF-R-alpha, GMCSFR-alpha, GMR-alpha, CSF2R, CSF2RY, CSF2RA, CDw116, CD116, CSF2RAX, CSF2RAY, CSF2RX, GMCSFR, GMR, SMDP4,In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.
分子量
37.7 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.