SARS-CoV-2 Spike Protein (RBD) (mFc Tag)
Quick Overview for SARS-CoV-2 Spike Protein (RBD) (mFc Tag) (ABIN7274398)
抗原
See all SARS-CoV-2 Spike 蛋白蛋白类型
宿主
资源
纯度
-
-
产品特性
- RBD
-
标记
- This SARS-CoV-2 Spike protein is labelled with mFc Tag.
-
原理
- SARS-COV-2 Spike RBD Protein
-
序列
- Arg319-Asn532
-
产品特性
- Recombinant SARS-COV-2 Spike RBD Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Arg319-Asn532.
-
过滤
- 0.22 μm filtered
-
内毒素水平
- Less than 1EU per μg by the LAL method.
-
Biological Activity Comment
- Immobilized SARS-COV-2 Spike RBD at 0.2μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 60.9ng/ml determined by ELISA. See testing image for detail.
-
-
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
-
缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
-
储存条件
- -20 °C,-80 °C
-
储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
-
有效期
- 12 months
-
-
- SARS-CoV-2 Spike
-
背景
- The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
-
分子量
- 49.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
抗原
-