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SARS-CoV-2 Spike Protein (RBD) (mFc Tag)

This Recombinant SARS-CoV-2 Spike protein is expressed in HEK-293 Cells.
产品编号 ABIN7274398
发货至: 中国

Quick Overview for SARS-CoV-2 Spike Protein (RBD) (mFc Tag) (ABIN7274398)

抗原

See all SARS-CoV-2 Spike 蛋白
SARS-CoV-2 Spike

蛋白类型

Recombinant

宿主

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SARS Coronavirus-2 (SARS-CoV-2)

资源

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HEK-293 Cells

纯度

> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
  • 产品特性

    RBD

    标记

    This SARS-CoV-2 Spike protein is labelled with mFc Tag.

    原理

    SARS-COV-2 Spike RBD Protein

    序列

    Arg319-Asn532

    产品特性

    Recombinant SARS-COV-2 Spike RBD Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Arg319-Asn532.

    过滤

    0.22 μm filtered

    内毒素水平

    Less than 1EU per μg by the LAL method.

    Biological Activity Comment

    Immobilized SARS-COV-2 Spike RBD at 0.2μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 60.9ng/ml determined by ELISA. See testing image for detail.
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  • 限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

    缓冲液

    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

    储存条件

    -20 °C,-80 °C

    储存方法

    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    SARS-CoV-2 Spike

    背景

    The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

    分子量

    49.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
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