SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 16-1188, Trimer) (His-Avi Tag)
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Quick Overview for SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 16-1188, Trimer) (His-Avi Tag) (ABIN7274406)
抗原
See all SARS-CoV-2 Spike (SARS-CoV-2 S) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 16-1188, Trimer
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标记
- This SARS-CoV-2 Spike protein is labelled with His-Avi Tag.
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原理
- Non-biotinylated SARS-COV-2 Spike S Trimer Protein
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序列
- Val16-Glu1188
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产品特性
- Recombinant SARS-COV-2 Spike S is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Glu1188 trimer design.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized SARS-COV-2 Spike S Trimer, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA.,SARS-COV-2 Spike S Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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SARS-CoV-2 Spike (SARS-CoV-2 S) (Super Stable Trimer) protein (His tag)
SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-532), (RBD) (Active) protein (His-Avi Tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-541), (B.1.1.529 - Omicron), (RBD) (Active) protein (His tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-541), (E484K), (K417N), (N501Y), (RBD) (Active) protein (His tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-541), (B.1.1.7 - alpha), (RBD) protein (Fc Tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. IA, AM, ADA, ScA
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-541), (B.1.617.2 - delta), (RBD) protein (Fc Tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. IA, AM, ADA, ScA
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-541), (RBD) protein (His tag,mFc Tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. IA, AM, ADA, ScA
SARS-CoV-2 Spike (SARS-CoV-2 S) (Super Stable Trimer) protein (His tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-541), (B.1.640.2), (RBD) (Active) protein (His tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 16-1188), (D614G), (Trimer) (Active) protein (His-Avi Tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- SARS-CoV-2 Spike (SARS-CoV-2 S)
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别名
- SARS-COV-2 Spike
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物质类
- Viral Protein
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背景
- The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
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分子量
- 136.6 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Bis-Tris PAGE result.
抗原
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