CLEC4A
(C-Type Lectin Domain Family 4, Member A (CLEC4A))
蛋白类型
Recombinant
产品特性
AA 70-237
宿主
人
资源
HEK-293 Cells
标记
This CLEC4A protein is labelled with Fc Tag.
原理
Human CLEC4A/DCIR Protein
序列
Gln70-Leu237
产品特性
Recombinant Human CLEC4A/DCIR Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln70-Leu237.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CLEC4A, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC4A Antibody, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA. See testing image for detail.
CLEC4A
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
CLEC4A
(C-Type Lectin Domain Family 4, Member A (CLEC4A))
CLEC4A Protein, CLECSF6 Protein, DCIR Protein, DDB27 Protein, LLIR Protein, Clecsf6 Protein, Dcir1 Protein, Clec4a Protein, zinc finger protein 705A Protein, C-type lectin domain family 4 member A Protein, C-type lectin domain family 4, member A Protein, C-type lectin domain family 4, member a2 Protein, ZNF705A Protein, CLEC4A Protein, Clec4a Protein, Clec4a2 Protein
背景
Clec4a has been reported to be an immune suppressor of dendritic cells (DCs), but its potential role in cancer therapy remains to be elucidated. silencing of Clec4a2 expression via skin delivery of shRNA produces an effective antitumor response and that Clec4a2 shRNA may have therapeutic potential as an adjuvant for cancer immunotherapy.
分子量
46.7 kDa. Due to glycosylation, the protein migrates to 60-65 kDa based on Tris-Bis PAGE result.