FCGR2B Protein (AA 40-217) (His tag)
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- 抗原 See all FCGR2B 蛋白
- FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))
- 蛋白类型
- Recombinant
- 产品特性
- AA 40-217
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This FCGR2B protein is labelled with His tag.
- 原理
- Mouse Fc gamma RIIB/CD32b Protein
- 序列
- Thr40-Arg217
- 产品特性
- Recombinant Mouse Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr40-Arg217.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 0.280 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (AA 46-217) protein (His-Avi Tag)
FCGR2B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (AA 46-224) protein (His tag)FCGR2B 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) protein (His-Avi Tag,Biotin)FCGR2B 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (AA 45-154) protein (GST tag)FCGR2B 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (AA 46-217) protein (His tag)FCGR2B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (Active) protein (His tag)FCGR2B 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (Active) protein (His tag)FCGR2B 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (Active) protein (Biotin,His-Avi Tag)FCGR2B 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (AA 43-217) protein (His tag)Crystallography grade FCGR2B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B) (AA 43-310) protein (rho-1D4 tag)Crystallography grade FCGR2B 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))
- 别名
- Fc gamma RIIB (FCGR2B 产品)
- 别名
- CD32 Protein, CD32B Protein, FCG2 Protein, FCGR2 Protein, IGFR2 Protein, FCGR2B Protein, AI528646 Protein, F630109E10Rik Protein, Fc[g]RII Protein, FcgRII Protein, Fcgr2 Protein, Fcgr2a Protein, Fcr-2 Protein, Fcr-3 Protein, Ly-17 Protein, Ly-m20 Protein, LyM-1 Protein, Lym-1 Protein, FCRII Protein, FcRII Protein, Fc fragment of IgG receptor IIb Protein, Fc fragment of IgG, low affinity IIb, receptor (CD32) Protein, Fc receptor, IgG, low affinity IIb Protein, low affinity immunoglobulin gamma Fc region receptor II Protein, FCGR2B Protein, Fcgr2b Protein, LOC100856270 Protein
- 背景
- The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains, these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
- 分子量
- 21.4 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result.
- NCBI登录号
- NP_001070657
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling
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