CD47 Protein (CD47) (AA 19-139) (His tag)
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- 抗原 See all CD47 蛋白
- CD47
- 蛋白类型
- Recombinant
- 产品特性
- AA 19-139
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CD47 protein is labelled with His tag.
- 原理
- Human CD47 Protein
- 序列
- Gln19-Pro139
- 产品特性
- Recombinant Human CD47 is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Pro139.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD47, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 28.9ng/ml determined by ELISA. The affinity constant of 18.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Discover our top product CD47 蛋白
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CD47 (CD47) (AA 19-139) protein (Fc Tag)
CD47 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-141) protein (His tag)CD47 宿主: 猕猴, Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-139) protein (His-Avi Tag,Biotin)CD47 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-140) protein (Fc Tag)CD47 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-140) protein (His tag)CD47 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) protein (mFc-His Tag)CD47 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD47 (CD47) (AA 19-139) (Active) protein (Fc Tag)CD47 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) (AA 19-139) (Active) protein (His tag)CD47 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) (AA 19-139) (Active) protein (Fc Tag,AVI tag,Biotin)CD47 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) (AA 19-139) (Active) protein (His tag,AVI tag)CD47 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD47
- 别名
- CD47 (CD47 产品)
- 别名
- IAP Protein, MER6 Protein, OA3 Protein, 9130415E20Rik Protein, AA407862 Protein, AI848868 Protein, AW108519 Protein, B430305P08Rik Protein, Itgp Protein, CD47/IAP Protein, alkaline phosphatase, intestinal Protein, CD47 molecule Protein, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) Protein, Cd47 molecule Protein, ALPI Protein, CD47 Protein, Cd47 Protein
- 背景
- CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
- 分子量
- 14.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
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