CD58 Protein (CD58) (AA 29-215) (Fc Tag)
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- 抗原 See all CD58 蛋白
- CD58
- 蛋白类型
- Recombinant
- 产品特性
- AA 29-215
- 宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CD58 protein is labelled with Fc Tag.
- 原理
- Human CD58 Protein
- 序列
- Phe29-Arg215
- 产品特性
- Recombinant Human CD58 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe29-Arg215.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 9.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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CD58 (CD58) (AA 1-240) protein (GST tag)
CD58 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
CD58 (CD58) (AA 29-215) protein (His tag)Crystallography grade CD58 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
CD58 (CD58) (AA 29-250) protein (rho-1D4 tag)Crystallography grade CD58 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
CD58 (CD58) (AA 29-215) (Active) protein (Fc Tag)CD58 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD58 (CD58) (AA 29-215) protein (His tag)CD58 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD58
- 别名
- CD58 (CD58 产品)
- 别名
- LFA-3 Protein, LFA3 Protein, ag3 Protein, CD58 molecule Protein, CD58 Protein
- 背景
- Loss of CD58 is a common mechanism for tumor immune evasion in lymphoid malignancies. CD58 loss is known to occur due to both genetic and non-genetic causes, therefore, we hypothesized that restoring CD58 expression in lymphoma cells may be an effective treatment approach. EZH2 is involved in the epigenetic silencing of CD58 in lymphoma cells as a mechanism for tumor immune escape, and EZH2 inhibitors are able to restore epigenetically suppressed CD58 expression.
- 分子量
- 48.1 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
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