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TNFRSF8 Protein (AA 19-379) (His-Avi Tag,Biotin)

TNFRSF8 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7274158
发货至: 中国
  • 抗原 See all TNFRSF8 蛋白
    TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
    蛋白类型
    Recombinant
    产品特性
    AA 19-379
    宿主
    • 24
    • 7
    • 2
    • 1
    资源
    • 15
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    HEK-293 Cells
    标记
    This TNFRSF8 protein is labelled with His-Avi Tag,Biotin.
    原理
    Biotinylated Human CD30/TNFRSF8 Protein
    序列
    Phe19-Lys379
    产品特性
    Recombinant Biotinylated Human CD30/TNFRSF8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe19-Lys379.
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Anti-CD30 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 49ng/ml determined by ELISA. See testing image for detail.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
    别名
    CD30 (TNFRSF8 产品)
    别名
    CD30 Protein, D1S166E Protein, Ki-1 Protein, Cd30 Protein, Ki Protein, TNFRSF8 Protein, TNF receptor superfamily member 8 Protein, tumor necrosis factor receptor superfamily, member 8 Protein, TNFRSF8 Protein, Tnfrsf8 Protein
    背景
    The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs).
    分子量
    41.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.
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